POTABLE WATER COLLECTION FOR CHEMICAL AND MICROBIOLOGY IN-FLIGHT AND POST-FLIGHT ANALYSIS - WMK/WS&A


Note
Water collection will be taken from three locations for Chemical and Microbiology analyses: SRV-K (hot water tap), SRV-K (cold water tap) and SVO-ZV. The entire collection procedure will be completed for each tap before proceeding to remaining taps.
1. 
1.  Unstow from Water Sampler and Archiver (WS&A): ISS Potable Water Collection Subpack (one), Sharpie Pen, Water Microbiology Kit (WMK)
Note
1. SRV-K water: Turn SRV-K heater on before collecting water samples. Start sampling only after heating cycle is completed. Each heating cycle requires 15 minutes for pasteurization of 525 mL of water. One delivery = 25 mL.
2. SVO-ZV: The hand pump may be used to provide sufficient pressure to permit water sample collection. There is no device for accurate SVO-ZV water amount measurement. Crewmember will be required to perform visual estimation of 25 mL of flush water and 100 mL and 125 mL samples by comparison to SRV-K samples.
Caution
To avoid contamination, use new potable water sampler for each tap.
2. 
1.  Wipe appropriate tap SRV-K (SVO-ZV) with Disinfectant Wipe. Discard Wipe.
3. 
1.  Remove one potable water sampler from Water Sampler & Archiver (WS&A) Subpack and remove from protective package.
2.  Place potable water sampler package in WS&A.
Caution
1. To avoid sample contamination, do not touch SRV-K (SVO-ZV) tap.
2. Hold potable water sampler by middle only.
4. 
1.  Put potable water sampler on SRV-K (SVO-ZV) tap.
5. 
ATTACHING SMALL WASTE WATER BAG
1.  Unstow one Small Waste Water Bag from WS&A Subpack.
2.  Remove cap from Small Waste Water Bag connection.
3.  Attach the Small Waste Water Bag to potable water sampler, bag connection ↷.
6. 
COLLECTING WATER INTO SMALL WASTE WATER (FLUSH) BAG
1.  Currently collecting from SRV-K (hot or cold)
2.  Switch WATER QUANTITY mL → CONTINUOUS
3.  Delivery valve (HOT or COLD) → OPEN
4.  ✓ DSPR RDY LED is lit
5.  Push H2O DSPR Pump pb → ON
6.  Place 25 mL of water (one cycle of dispenser) into Small Waste Water Bag (1/12 full).
7.  Push H2O DSPR Pump pb → OFF when desired amount of H2O has been dispensed
8.  Delivery valve (HOT or COLD) → CLOSE (after each portion collection)
9.  Place 25 mL of water into Small Waste Water Bag (1/12 full).
Note
1. Do not remove Small Waste Water Bag until ready to connect TOC Water Sample Bag or Syringe.
2. Do not perform collection into TOC Sample Syringe unless instructed to.
7. 
1.  Unstow Water Sample Bag or TOC Sample Syringe from WS&A Subpack.
8. 
1.  Record date, time, and sampling location on Bag or Syringe. Label using Sharpie Pen.
Caution
Do not remove potable water sampler from port until all samples have been collected from that port.
9. 
REMOVING SMALL WASTE WATER BAG
1.  Remove Small Waste Water Bag from potable water sampler, bag connection ↶.
2.  Replace cap onto Small Waste Water Bag connection.
3.  Place the Small Waste Water Bag into a small Ziploc Storage Bag.
4.  Stow in Subpack (WS&A).
10. 
ATTACHING TOC WATER SAMPLE BAG OR SYRINGE TO POTABLE WATER SAMPLER CONNECTION
1.  Attaching the Bag
2.  Remove cap from TOC Water Sample Bag connection.
3.  Attach TOC Water Sample Bag to potable water sampler, bag connection ↷.
4.  ✓ Connection is tight
Note
Do not overfill bag. Overfilled bag may cause leakage.
5.  Remove cap from TOC Sample Syringe connection.
6.  Attach TOC Sample Syringe to potable water sampler, Syringe connection ↷.
7.  ✓ Connection is tight
8.  OPEN SYRINGE SHUTOFF VALVE. Turn valve to position that is parallel to flow direction.
11. 
COLLECTING WATER INTO TOC WATER SAMPLE BAG OR SYRINGE
1.  Collection into Bag
2.  Collection from SRV-K (hot or cold)
3.  Switch WATER QUANTITY mL → CONTINUOUS
4.  Delivery valve (HOT or COLD) → OPEN
5.  ✓ DSPR RDY LED is lit
6.  Push H2O DSPR Pump pb → ON
7.  Place 100 mL of water (four deliveries) into TOC Water Sample Bag (1/3 full).
8.  Push H2O DSPR Pump pb → OFF when desired amount of H2O has been dispensed
9.  Delivery valve (HOT or COLD) → CLOSE (after each portion collection)
10.  Place 100 mL of water into TOC Water Sample Bag (1/3 full).
11.  Collecting from SRV-K (hot or cold)
12.  Switch WATER QUANTITY mL → CONTINUOUS
13.  Delivery valve (HOT or COLD) → OPEN
14.  ✓ DSPR RDY LED is lit
15.  Push H2O DSPR Pump pb → ON
Note
Plunger is designed so that it cannot be withdrawn beyond 25 mL mark. Do not underfill Syringe.
16.  Place 25 mL of water (one cycle of dispenser) into TOC Water Sample Syringe.
17.  Push H2O DSPR Pump pb → OFF when desired amount of H2O has been dispensed
18.  Delivery valve (HOT or COLD) → CLOSE (after each portion collection)
19.  Turn Syringe vlv perpendicular to flow direction.
Note
Plunger is designed so that it cannot be withdrawn beyond 25 mL mark. Do not underfill Syringe.
20.  Place 25 mL of water into TOC Sample Syringe.
21.  Turn syringe valve perpendicular to flow direction.
Note
Do not remove TOC Water Sample Bag or Syringe until ready to connect Chemical Post-Flight Analysis Bag.
12. 
1.  Unstow Chemical Sample Post-Flight Analysis Bag from WS&A Subpack.
2.  Record date, time, and sampling location on Chemical Sample Post-Flight Analysis Bag Label using Sharpie Pen.
Caution
Do not remove potable water sampler from port until all samples have been collected from that port.
13. 
REMOVING TOC WATER SAMPLE BAG OR SYRINGE/ATTACHING CHEMICAL SAMPLE POST-FLIGHT ANALYSIS BAG
1.  Collecting in BAG
2.  Remove TOC Water Sample Bag from potable water sampler, bag connection ↶.
3.  Replace cap on bag.
4.  Remove cap from Chemical Sample Post-Flight Analysis Bag connection
5.  Attach Chemical Sample Post-Flight Analysis Bag to water potable water sampler, bag connection ↷.
6.  Place TOC Water Sample Bag into small Ziploc Storage Bag.
7.  Temporary stow Sample Bag in WS&A
8.  Remove TOC Sample Syringe from potable water sampler, bag connection ↶.
9.  Replace cap on syringe connection.
10.  Attach Chemical Sample Post-Flight Analysis Bag to water potable water sampler, bag connection ↷.
11.  Replace cap on syringe connection.
12.  Place Syringe into small Ziploc Storage Bag.
13.  Temporary stow in WS&A.
14. 
COLLECTING WATER INTO CHEMICAL SAMPLE POST-FLIGHT ANALYSIS BAG
1.  Currently collecting from SRV-K (hot or cold)
2.  Switch WATER QUANTITY mL → CONTINUOUS
3.  Delivery valve (HOT or COLD) → OPEN
4.  ✓ DSPR RDY LED is lit
5.  Push H2O DSPR Pump pb → ON
6.  Place 750 mL of water (30 deliveries) into Chemical Sample Post-Flight Analysis Bag (3/4 full).
7.  Push H2O DSPR Pump pb → OFF when desired amount of H2O has been dispensed
8.  Delivery valve (HOT or COLD) → CLOSE (after each portion collection)
9.  Place 750 mL of water into Chemical Sample Post-Flight Analysis Bag (3/4 full).
Note
Do not remove Chemical Sample Post-Flight Analysis Bag until ready to connect Micro-Sample In-Flight Analysis Bag.
15. 
1.  Unstow Micro-Sample In-Flight Analysis Bag from WMK Subpack.
2.  Record date and circle sampling location on Micro-Sample In-Flight Analysis Bag using Sharpie Pen.
Caution
Do not remove potable water sampler from port until all samples have been collected from that port.
16. 
REMOVING CHEMICAL SAMPLE POST-FLIGHT ANALYSIS BAG
1.  Remove Chemical Sample Post-Flight Analysis Bag from potable water sampler, bag connection ↶.
2.  Replace cap on bag.
3.  Place bag into large Ziploc Storage Bag.
4.  Temporary stow bag in TBD.
17. 
ATTACHING MICRO-SAMPLE IN-FLIGHT ANALYSIS BAG
1.  Remove cap from Micro-Sample In-Flight Analysis Bag.
2.  Attach Micro-Sample In-Flight Analysis Bag to potable water sampler, bag connection ↷.
Note
Do not overfill bag. Overfilled bag may cause leakage.
18. 
COLLECTING WATER INTO MICRO-SAMPLE IN-FLIGHT ANALYSIS BAG
1.  Currently collecting from SRV-K (hot or cold)
2.  Switch WATER QUANTITY mL → CONTINUOUS
3.  Delivery valve (HOT or COLD) → OPEN
4.  ✓ DSPR RDY LED is lit
5.  Push H2O DSPR Pump pb → ON
6.  Place 125 mL of water (five deliveries) into Micro-Sample In-Flight Bag (3/4 full).
7.  Push H2O DSPR Pump pb → OFF when desired amount of H2O has been dispensed
8.  Delivery valve (HOT or COLD) → CLOSE (after each portion collection)
9.  Place 125 mL of water into Micro-Sample In-Flight Bag (1/2 full).
Note
Do not remove Micro-Sample In-Flight Analysis Bag until ready to connect Micro-Sample Post-Flight Analysis Bag.
19. 
1.  Unstow Micro-Sample Post-Flight Analysis Bag from WMK Subpack.
2.  Record date and circle sampling location on Bag using Sharpie Pen.
Caution
Do not remove potable water sampler from port until all samples have been collected from that port.
20. 
REMOVING MICRO-SAMPLE IN-FLIGHT ANALYSIS BAG
1.  Remove Micro-Sample In-Flight Analysis Bag from potable water sampler, bag connection ↶.
2.  Replace cap on bag connection.
3.  Temporary stow bag in WMK.
21. 
1.  Remove cap from Micro-Sample Post-Flight Analysis Bag.
2.  Attach Micro-Sample Post-Flight Analysis Bag to water sampler, bag connection ↷.
22. 
COLLECTING WATER INTO MICRO-SAMPLE POST-FLIGHT ANALYSIS BAG
1.  Currently collecting from SRV-K (hot or cold)
2.  Switch WATER QUANTITY mL → CONTINUOUS
3.  Delivery valve (HOT or COLD) → OPEN
4.  ✓ DSPR RDY LED is lit
5.  Push H2O DSPR Pump pb → ON
6.  Place 1000 mL of water (40 deliveries) into Micro-Sample Post-Flight Bag (full).
7.  Push H2O DSPR Pump pb → OFF when desired amount of H2O has been dispensed
8.  Delivery valve (HOT or COLD) → CLOSE (after each portion collection)
9.  Place 1000 mL of water into Micro-Sample Post-Flight Bag (full).
23. 
REMOVING MICRO-SAMPLE POST-FLIGHT ANALYSIS BAG
1.  Remove Micro-Sample Post-Flight Analysis Bag from potable water sampler, bag connection ↶.
2.  Replace cap on bag.
3.  Place bag into large Ziploc Storage Bag (from WMK Subpack).
4.  Temporary stow bag in WMK.
24. 
1.  Remove potable water sampler from SRV-K (SVO-ZV) tap.
25. 
1.  Place potable water sampler into small Ziploc Storage Bag. Place in WS&A Subpack.
26. 
1.  Are there still other ports requiring sampling ?
27. 
1.  Place Subpack into WS&A
28. 
1.  Stow WS & A
1 Water Collection Order - Chemical and Micro In-Flight and Post-Flight (photo)
2 Small Waste Water Bag (photo)
3 TOC Water Sample Bag (photo)
4 Chemical Sample Post-Flight Analysis Bag (photo)
5 Micro-Sample In-Flight Analysis Bag (photo)
6 Micro-Sample Post-Flight Analysis Bag (photo)